| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Prevotella ruminicola | TGCACAGGGTAAGTTCGGC | AGCCGAAGCCTTCTGCAA | 60.30 | 59.57 | 283 | 108 |
100.00%
|
100.00%
|
| Primer 42 | Prevotella ruminicola | TCACGCCACTCTTCGCAA | TCGAGCGAACGACTTGCA | 59.58 | 59.67 | 171 | 108 |
100.00%
|
100.00%
|
| Primer 43 | Prevotella ruminicola | ATCACGCCACTCTTCGCAA | TCGAGCGAACGACTTGCA | 60.01 | 59.67 | 172 | 108 |
100.00%
|
100.00%
|
| Primer 44 | Prevotella ruminicola | TTGTGGCTGCGCTGGTAA | AGCAACTCACGAACGGCA | 59.89 | 59.89 | 232 | 107 |
100.00%
|
100.00%
|
| Primer 45 | Prevotella ruminicola | TCATTCCCGACACCGCAA | AATCGCCAAGACGTCGCT | 59.26 | 59.74 | 188 | 106 |
100.00%
|
100.00%
|
| Primer 46 | Prevotella ruminicola | AGCAGACCGTGTGTTGGT | AATCGCCAAGACGTCGCT | 59.09 | 59.74 | 244 | 106 |
100.00%
|
100.00%
|
| Primer 47 | Prevotella ruminicola | ATCATTCCCGACACCGCA | AATCGCCAAGACGTCGCT | 59.01 | 59.74 | 189 | 106 |
100.00%
|
100.00%
|
| Primer 48 | Prevotella ruminicola | TCAAAGCGTCACCGTGGT | AGTACCAGACGAGAGCGGT | 59.50 | 60.00 | 290 | 106 |
100.00%
|
100.00%
|
| Primer 49 | Prevotella ruminicola | TCAAAGCGTCACCGTGGT | AGTTGCAGGCGTTGGTACA | 59.50 | 59.85 | 226 | 106 |
100.00%
|
100.00%
|
| Primer 50 | Prevotella ruminicola | TCAAAGCGTCACCGTGGT | TGCAGGCGTTGGTACACAA | 59.50 | 60.15 | 223 | 106 |
100.00%
|
100.00%
|